HGD antibody (AA 374-445)

Catalog No. ABIN5693116

The Rabbit Polyclonal anti-HGD antibody has been validated for WB, IHC, ELISA, ICC, IF and FACS. It is suitable to detect HGD in samples from Human, Mouse and Rat.

Quick Overview for HGD antibody (AA 374-445) (ABIN5693116)

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HGD Antibody

Binding Specificity: AA 374-445

Purpose: Anti-HGD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HGD Antibody Picoband® (ABIN5693116). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human HGD recombinant protein (Position: D374-N445).

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section),0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA,0.1-0.5 μg/mL
1. Fernandez-Canon, J. M., Granadino, B., Beltran-Valero de Bernabe, D., Renedo, M., Fernandez-Ruiz, E., Penalva, M. A., Rodriguez de Cordoba, S. The molecular basis of alkaptonuria. Nature Genet. 14: 19-24, 1996. 2. Vilboux, T., Kayser, M., Introne, W., Suwannarat, P., Bernardini, I., Fischer, R., O'Brien, K., Kleta, R., Huizing, M., Gahl, W. A. Mutation spectrum of homogentisic acid oxidase (HGD) in alkaptonuria. Hum. Mutat. 30: 1611-1619, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HGD

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Alternative Name: HGD

Background:

Synonyms: Homogentisate 1,2-dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase, HGD, HGO

Tissue Specificity: Highest expression in the prostate, small intestine, colon, kidney and liver.

Background: The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.

Molecular Weight: 50 kDa

Gene ID: 3081

UniProt: Q93099