NPC1 antibody (AA 1022-1278)

Catalog No. ABIN5693129

The Rabbit Polyclonal anti-NPC1 antibody (ABIN5693129) specifically detects NPC1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NPC1 antibody (AA 1022-1278) (ABIN5693129)

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NPC1 Antibody

Binding Specificity: AA 1022-1278

Purpose: Anti-Niemann Pick C1/NPC1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Niemann Pick C1/NPC1 Antibody (ABIN5693129). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Immunogen: E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278).

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL
ELISA,0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NPC1

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Alternative Name: NPC1

Background:

Synonyms: Niemann-Pick C1 protein, NPC1

Background: Niemann-Pick disease, type C1 (NPC1) is a membrane protein that mediates intracellular cholesterol trafficking in mammals. In humans it is encoded by the NPC1 gene (chromosome location 18q11). This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.

Molecular Weight: 170 kDa

Gene ID: 4864

UniProt: O15118