TLR1 antibody (AA 350-404) (DyLight 488)

Catalog No. ABIN7354510

This Rabbit Polyclonal antibody specifically detects TLR1 in FACS. It exhibits reactivity toward Human.

Quick Overview for TLR1 antibody (AA 350-404) (DyLight 488) (ABIN7354510)

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR1 antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Product Details anti-TLR1 Antibody (DyLight 488)

Binding Specificity: AA 350-404

Purpose: Anti-Human TLR1 DyLight® 488 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human TLR1 DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: E. coli-derived human TLR1 recombinant protein (Position: F350-D404).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Hart, B. E., Tapping, R. I. Differential trafficking of TLR1 I602S underlies host protection against pathogenic mycobacteria. J. Immun. 189: 5347-5355, 2012. 2. West, A. P., Brodsky, I. E., Rahner, C., Woo, D. K., Erdjument-Bromage, H., Tempst, P., Walsh, M. C., Choi, Y., Shadel, G. S., Ghosh, S. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476-480, 2011. 3. Xu, Y., Tao, X., Shen, B., Horng, T., Medzhitov, R., Manley, J. L., Tong, L. Structural basis for signal transduction by the Toll/interleukin-1 receptor domains. Nature 408: 111-115, 2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for TLR1

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Alternative Name: TLR1

Background:

Synonyms: Toll-like receptor 1, Toll/interleukin-1 receptor-like protein, TIL, CD281, TLR1, KIAA0012

Tissue Specificity: Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

Background: Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

Molecular Weight: 39 kDa

Gene ID: 7096

UniProt: Q15399

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades