AGRP antibody (AA 82-131)

Catalog No. ABIN5693160

This Rabbit Polyclonal antibody specifically detects AGRP in IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for AGRP antibody (AA 82-131) (ABIN5693160)

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRP antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-AGRP Antibody

Binding Specificity: AA 82-131

Purpose: Anti-AGRP Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AGRP Antibody Picoband® (ABIN5693160). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Immunogen: E. coli-derived mouse AGRP recombinant protein (Position: S82-T131).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Ollmann MM, Wilson BD, Yang YK, Kerns JA, Chen Y, Gantz I, Barsh GS (Oct 1997). "Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein". Science. 278 (5335): 135-8. 2. Shutter JR, Graham M, Kinsey AC, Scully S, Lüthy R, Stark KL (Mar 1997). "Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice". Genes & Development.11 (5): 593-602.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Alternative Name: Agrp

Background:

Synonyms: Agouti-related protein, Agrp, Agrt, Art

Tissue Specificity: Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Background: Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.

Molecular Weight: 49 kDa

Gene ID: 11604

UniProt: P56473

Pathways: Feeding Behaviour, Photoperiodism