Lactoferrin antibody (AA 529-710)

Catalog No. ABIN5693189

This anti-Lactoferrin antibody is a Rabbit Polyclonal antibody detecting Lactoferrin in WB, IHC, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Lactoferrin antibody (AA 529-710) (ABIN5693189)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Lactoferrin Antibody

Binding Specificity: AA 529-710

Purpose: Anti-Lactoferrin/LTF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lactoferrin/LTF Antibody Picoband® (ABIN5693189). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Lactoferrin recombinant protein (Position: D529-K710).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Jing-Fen Kang, Xiang-Long Li, Rong-Yan Zhou, Lan-Hui Li, Fu-Jun Feng, Xiu -Li Guo (2008). "Bioinformatics Analysis of Lactoferrin Gene for Several Species". Biochemical Genetics. 46 (5-6): 312-322. 2. Sánchez L, Calvo M, Brock JH (1992). "Biological role of lactoferrin". Arch. Dis. Child. 67 (5): 657-61.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: LTF

Background:

Synonyms: Lactotransferrin, Lactoferrin, Growth-inhibiting protein 12, Talalactoferrin, Lactoferricin-H, Lfcin-H, Kaliocin-1, Lactoferroxin-A, Lactoferroxin-B, Lactoferroxin-C, LTF, GIG12, LF

Tissue Specificity: High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level). Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver. Isoform 1 is expressed in brain, testis and peripheral blood leukocytes, isoform DeltaLf is barely detectable in these tissues. Isoform DeltaLf is expressed in placenta, liver and ovary, isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.

Background: Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides.

Molecular Weight: 85 kDa

Gene ID: 4057

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis