ELF1 antibody

Catalog No. ABIN5693215

The Rabbit Polyclonal anti-ELF1 antibody has been validated for WB. It is suitable to detect ELF1 in samples from Human, Mouse and Rat.

Quick Overview for ELF1 antibody (ABIN5693215)

Target: ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ELF1 Antibody

Purpose: Anti-E74 like factor 1/ELF1 Antibody Picoband®

Sequence: QPTQSPYPTQ LFRTVHVVQP VQAVPEGEAA RTSTMQDE

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-E74 like factor 1/ELF1 Antibody Picoband® (ABIN5693215). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E74 like factor 1, which shares 81.6% amino acid (aa) sequence identity with both mouse and rat E74 like factor 1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
1. Aryee, D. N. T., Petermann, R., Kos, K., Henn, T., Haas, O. A., Kovar, H. Cloning of a novel human ELF-1-related ETS transcription factor, ELFR, its characterization and chromosomal assignment relative to ELF-1. Gene 210: 71-78, 1998. 2. Hollenhorst, P. C., Shah, A. A., Hopkins, C., Graves, B. J. Genome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene family.Genes Dev. 21: 1882-1894, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ELF1

Target: ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))

Alternative Name: ELF1

Background:

Synonyms: ETS-related transcription factor Elf-1, E74-like factor 1, ELF1

Tissue Specificity: In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.

Background: E74-like factor 1 (ets domain transcription factor) is a protein that in humans is encoded by the ELF1 gene. It is mapped to chromosome 13q13. This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants.

Molecular Weight: 75 kDa

Gene ID: 1997

UniProt: P32519