EOMES antibody (AA 310-532)

Catalog No. ABIN5693217

This Rabbit Polyclonal antibody specifically detects EOMES in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EOMES antibody (AA 310-532) (ABIN5693217)

Target: EOMES (Eomesodermin (EOMES))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EOMES antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EOMES Antibody

Binding Specificity: AA 310-532

Purpose: Anti-TBR2/Eomes Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TBR2/Eomes Antibody Picoband® (ABIN5693217). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human TBR2/Eomes recombinant protein (Position: T310-D532).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Arnold SJ, Huang GJ, Cheung AF, Era T, Nishikawa S, Bikoff EK, Molnár Z, Robertson EJ, Groszer M (2008). "The T-box transcription factor Eomes/Tbr2 regulates neurogenesis in the cortical subventricular zone". Genes & Development. 22 (18): 2479-84. 2. Sessa A, Mao CA, Hadjantonakis AK, Klein WH, Broccoli V (Oct 2008). "Tbr2 s conversion of radial glia into basal precursors and guides neuronal amplification by in neurogenesis in the developing neocortex glish". Neuron. 60 (1): 56-69.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EOMES

Target: EOMES (Eomesodermin (EOMES))

Alternative Name: EOMES

Background:

Synonyms: Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2, EOMES, TBR2

Tissue Specificity: Expressed in CD8+ T-cells.

Background: Eomesodermin also known as T-box brain protein 2 (Tbr2) is a protein that in humans is encoded by the EOMES gene. This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants.

Molecular Weight: 85 kDa

Gene ID: 8320

UniProt: O95936

Pathways: Stem Cell Maintenance