PI3 antibody (AA 61-117)

Catalog No. ABIN5693295

This Mouse Monoclonal antibody specifically detects PI3 in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PI3 antibody (AA 61-117) (ABIN5693295)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: C7

Product Details anti-PI3 Antibody

Binding Specificity: AA 61-117

Purpose: Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Elafin/Skalp PI3 Antibody Picoband® (monoclonal, C7) (ABIN5693295). Tested in ELISA, IHC applications. This antibody reacts with Human.

Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA (Cap), 1-5 μg/mL1. "Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP)". 2. Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein". Biochem J 368 (Pt 1): 233-42. 3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13". Cytogenet Cell Genet 66 (2): 129-31.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: PI3

Background:

Synonyms: Elafin, Elastase-specific inhibitor, ESI, Peptidase inhibitor 3, PI-3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, SKALP, WAP four-disulfide core domain protein 14, PI3, WAP3, WFDC14

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Molecular Weight: 25 kDa

Gene ID: 5266

UniProt: P19957