AMBP antibody (AA 206-352)

Catalog No. ABIN5693314

The Rabbit Polyclonal anti-AMBP antibody has been validated for WB and ELISA. It is suitable to detect AMBP in samples from Human, Rat and Mouse.

Quick Overview for AMBP antibody (AA 206-352) (ABIN5693314)

Target: AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AMBP Antibody

Binding Specificity: AA 206-352

Purpose: Anti-Bikunin/AMBP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Bikunin/AMBP Antibody Picoband® (ABIN5693314). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Bikunin/AMBP recombinant protein (Position: A206-N352).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: AMBP alpha-1-microglobulin/bikunin precursor". 2. Salier JP, Simon D, Rouet P, Raguenez G, Muscatelli F, Gebhard W, Guenet JL, Mattei MG (Dec 1992). "Homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse: assignment of the ancestral gene for the lipocalin superfamily". Genomics. 14 (1): 83-8. 3. Vetr H, Gebhard W (Jun 1991). "Structure of the human alpha 1-microglobulin-bikunin gene". Biol. Chem. Hoppe-Seyler. 371 (12): 1185-96.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AMBP

Target: AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Alternative Name: AMBP

Background:

Synonyms: Protein AMBP, Alpha-1-microglobulin, Protein HC, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin, AMBP, HCP, ITIL

Tissue Specificity: Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Background: Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. Human bikunin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and the C-terminal serine protease inhibitor bikunin (aa 206-352).

Molecular Weight: 34 kDa

Gene ID: 259

UniProt: P02760