ALPL antibody

Catalog No. ABIN5696924

The Rabbit Polyclonal anti-ALPL antibody is suitable to detect ALPL in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for ALPL antibody (ABIN5696924)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-ALPL Antibody

Purpose: ALPL antibody

Immunogen: alkaline phosphatase, liver/bone/kidney

Isotype: IgG

Application Details

Application Notes: WB :1:500-1:5000,IHC:1:20-1:200

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00347(ALPL antibody) at dilution of 1:800

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background:

Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP, TNS-ALP, TNSALP)|Alkaline phosphatase liver/bone/kidney isozyme|Phosphoamidase|Phosphocreatine phosphatase|ALPL

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney(tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66- kDa endo-b-N-acetylglucos-aminidase H(Endo H)-sensitive form, and processed to an 80- kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol(GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.

Molecular Weight: 57kDa

Gene ID: 249

UniProt: P05186