alpha Actinin antibody

Catalog No. ABIN5697056

The Rabbit Polyclonal anti-alpha Actinin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect alpha Actinin in samples from Human, Mouse and Rat.

Quick Overview for alpha Actinin antibody (ABIN5697056)

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Actinin Antibody

Immunogen: actinin, alpha 1

Isotype: IgG

Application Details

Application Notes: WB : 1:1000-1:10000 IHC : 1:50-1:500 IF : 1:50-1:500

Comment:

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab00322( a-Actinin Antibody) at dilution of 1:2000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for alpha Actinin

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Alternative Name: alpha Actinin

Background: Synonyms:actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1 Background:Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 103 kDa

UniProt: P12814

Pathways: Cell-Cell Junction Organization, Negative Regulation of Transporter Activity