PKM1 antibody

Catalog No. ABIN5697233

The Rabbit Polyclonal anti-PKM1 antibody has been validated for WB, IHC, ELISA, IP and IF. It is suitable to detect PKM1 in samples from Human, Mouse and Rat.

Quick Overview for PKM1 antibody (ABIN5697233)

Target: PKM1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details

Purpose: PKM1-specific antibody

Immunogen: peptide

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06494(PKM1-specific) at dilution of 1:1500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details

Target: PKM1

Background:

Synonyms: PKM1|PK-M1|PKM1 pyruvate kinase|muscle|PKM|Pyruvate kinase PKM

Background: PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1, however, the primary isoform from four independent tumours is PK-M2(PMID:18337823). This antibody is specific to PKM1 isoform.