ube3a antibody

Catalog No. ABIN5698660

This Rabbit Polyclonal antibody specifically detects ube3a in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ube3a antibody (ABIN5698660)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-ube3a Antibody

Immunogen: ubiquitin protein ligase E3A

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000,IHC:1:20-1:200,IP:1:200-1:1000,IF:1:10-1:100

Comment:

mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab09189(UBE3A antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: UBE3A

Background: Synonyms:ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HPVE6A, UBE3A, ubiquitin protein ligase E3A Background:This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Molecular Weight: 38kDa,Observed 45kDa

Gene ID: 7337

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway