MMP13 antibody

Catalog No. ABIN5701112

The Rabbit Polyclonal anti-MMP13 antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect MMP13 in samples from Human, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for MMP13 antibody (ABIN5701112)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MMP13 Antibody

Immunogen: matrix metallopeptidase 13 (collagenase 3)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with FNab05235( MMP13 Antibody) at dilution of 1:600

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP13

Background: Synonyms:CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13 Background:Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.It can detect the glycosylated proMMP-13(70 kDa), unglycosylated proform(54 kDa).

Molecular Weight: 50-52 kDa

Gene ID: 4322

UniProt: P45452