PARD3 antibody (AA 180-442)

Catalog No. ABIN5704096

This Rabbit Polyclonal antibody specifically detects PARD3 in WB, ELISA, IHC, FACS and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PARD3 antibody (AA 180-442) (ABIN5704096)

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-PARD3 Antibody

Binding Specificity: AA 180-442

Purpose: PARD3 antibody

Immunogen:

Immunogen sequence: 180-442aa

Immunogen: Partitioning defective 3 homolog

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06146(PARD3 antibody) at dilution of 1:300

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PARD3

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: PARD3

Background:

Synonyms: Partitioning defective 3 homolog (PAR-3, PARD-3)|Atypical PKC isotype-specific-interacting protein (ASIP)|CTCL tumor antigen se2-5|PAR3-alpha|PARD3|PAR3|PAR3A

Background: Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination(By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Gene ID: 56288

UniProt: Q8TEW0

Pathways: Cell-Cell Junction Organization