Hemoglobin Subunit beta-C (LOC100134870) (N-Term) antibody

Catalog No. ABIN5706736

Supplier Product No.: 200-301-gs7

This Mouse Monoclonal antibody specifically detects in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for Hemoglobin Subunit beta-C (LOC100134870) (N-Term) antibody (ABIN5706736)

Target: Hemoglobin Subunit beta-C (LOC100134870)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: 15C2-C11-F2-G11

Product Details

Binding Specificity: N-Term

Supplier Product No.: 200-301-gs7

Supplier: Rockland

Purpose: Hemoglobin beta C Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbC beta c-variant isoform.

Characteristics: Synonyms: mouse anti-HbC antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit C variant, HbBc, HbC, HbC Antibody, LVV-hemorphin-7, Spinorphin, Beta-globin, Hemoglobin beta chain, Sickle Cell Disease (SCD)

Purification: Anti-HbC is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta C Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: Anti-Hemoglobin beta C (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Hemoglobin Subunit beta-C (LOC100134870)

Alternative Name: HBBc

Background: Background: HbC antibodies detect the E6K mutant in the hemoglobin beta subunit. Functional hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA2. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Less significant than the SCD-E6V, HbC E6K mutation causes a mild hemolytic anemia. HbC antibody does not react to other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

UniProt: P68871