POLQ antibody (Internal Region)

Catalog No. ABIN570678

This anti-POLQ antibody is a Goat Polyclonal antibody detecting POLQ in ELISA. Suitable for Human.

Quick Overview for POLQ antibody (Internal Region) (ABIN570678)

Target: POLQ (Polymerase (DNA Directed), theta (POLQ))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POLQ antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-POLQ Antibody

Binding Specificity: Internal Region

Purpose: DNA polymerase theta

Sequence: DTHQQLTCSN DES

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DTHQQLTCSNDES, from the internal region of the protein sequence according to NP_955452.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POLQ

Target: POLQ (Polymerase (DNA Directed), theta (POLQ))

Alternative Name: POLQ

Background: POLQ , polymerase (DNA directed), theta , DKFZp781A0112, POLH, PRO0327, DNA polymerase eta, DNA polymerase theta

Gene ID: 10721

NCBI Accession: NP_955452