Histone 3 antibody (H3R2me2a)

Catalog No. ABIN5706791

Supplier Product No.: 600-401-i53

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3R2me2a

Reactivity: Human, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-i53

Supplier: Rockland

Purpose: Histone H3 R2me2a Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H3.

Characteristics: Synonyms: rabbit anti-Histone H3 Asym-dimethyl Arg2 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R2me2a

Purification: Anti-Histone H3 [Asym-dimethyl Arg2] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Asym-dimethyl Arg2] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Arginine 2 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50-1:100

Application Note: Anti-Histone H3 [Asym-dimethyl Arg2] antibody is tested by Western Blot, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:50-1:100

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.71 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Background: When Set1 attempts to methylate H3K4, epigenetic silencing of downstream genes is mediated by dimethylation of histone H3 at Arg2. This modification is typically found in heterochromatin and inactive genes, and is not found when trimethylation of Arg2 is observed. Conversion from mono-, di- and tri-methyl forms of H3R2 is mediated through the activity of the arginine methyltransferase PRMT6. H3K4 cannot be methylated when the asymmetric H3R2me2 mark is present. In active promoters, this modification is actively eliminated. H3R2me2a prevents WDR5 recognition, MLL methyltransferase recruitment, and H3K4 methylation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3