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Histone H4 antibody (2meLys20)

This anti-Histone H4 antibody is a Rabbit Polyclonal antibody detecting Histone H4 in WB, IHC, IF, ChIP, DB and FM. Suitable for Human and C. elegans.
Rockland
Catalog No. ABIN5706798
Supplier Product No.: 600-401-j02

Quick Overview for Histone H4 antibody (2meLys20) (ABIN5706798)

Target

See all Histone H4 Antibodies
Histone H4

Reactivity

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Human, C. elegans

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone H4 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)
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    2meLys20

    Supplier Product No.

    600-401-j02

    Supplier

    Rockland

    Purpose

    Histone H4 K20me2 Antibody

    Cross-Reactivity (Details)

    This antibody reacts with human Histone H4.

    Characteristics

    Synonyms: rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

    Purification

    Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    Sterility

    Sterile filtered

    Immunogen

    Immunogen: Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.

    Immunogen Type: Conjugated Peptide

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:50

    Application Note: Anti-Histone H4 [Dimethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

    ChIP Dilution: 2-5 μg/million cells

    Western Blot Dilution: 1:2000

    IF Microscopy Dilution: 1:50

    Other: Dot Blot 1:1000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.53 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 30 % Glycerol

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Expiry Date

    12 months
  • Target

    Histone H4

    Background

    Background: When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.

    Gene ID

    121504

    NCBI Accession

    NP_001029249

    UniProt

    P62805
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