O3FAR1 antibody (N-Term)

Catalog No. ABIN5706869

Supplier Product No.: 600-401-gw0

The Rabbit Polyclonal anti-O3FAR1 antibody has been validated for WB and ELISA. It is suitable to detect O3FAR1 in samples from Mouse and Rat.

Quick Overview for O3FAR1 antibody (N-Term) (ABIN5706869)

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This O3FAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-O3FAR1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-gw0

Supplier: Rockland

Purpose: Ffar4 Antibody

Cross-Reactivity (Details): Anti-Ffar4 is directed against mouse Ffar4 at a n-terminal position.

Characteristics: Synonyms: rabbit anti-Ffar4 antibody, Ffar-4, Ffar 4, free fatty acid receptor 4, omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor GT01,  Gpr120, O3far1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Ffar4 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~40-48 kDa in size corresponding to Ffar4 by western blotting in the appropriate cell lysate or extract. Tested using positive controls Raw 264.7 WCL p/n W10-001-369 and NIH/3T3 WCL p/n W10-000-358.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for O3FAR1

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Alternative Name: Ffar4

Background: Background: Anti-Ffar4 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Free Fatty Acid 4 receptor (Ffa4 receptor or GPR120), a rhodopsin-like G protein coupled receptor (GPCR) subfamily member, is a receptor that senses specific fatty acids such as omega-3 fatty acid in fish oil or the endogenous signaling lipid, PHASA. Ffa4 receptor is enriched in lung, colon and adipose tissue but is also detected in many other tissues and cells. The activation of Ffar4 has multiple effects, including but not limited to inhibition of inflammation, improving insulin sensitivity and adipogenesis, and regulating hormone secretion from the gastro-intestinal system and pancreatic islets. Therefore, approaches that regulate FFA4 receptor activity could be developed as promising anti-diabetic and anti-inflammation drugs. GPR120 is the only fatty acid receptor that can sense lipids in adipose tissue, mature adipocytes, CD11c+ macrophages, and RAW264.7 cells making this receptor of potential importance in the prevention and treatment of metabolic and inflammatory diseases.

Gene ID: 107221

NCBI Accession: NP_861413

UniProt: Q7TMA4

Pathways: Hormone Transport