O3FAR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886324

The Rabbit Polyclonal anti-O3FAR1 antibody (ABIN7886324) specifically detects O3FAR1 in WB, IHC, FACS and LCI. The antibody is reactive with Mouse samples.

Quick Overview for O3FAR1 antibody (Extracellular, N-Term) (ABIN7886324)

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This O3FAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-O3FAR1 Antibody

Binding Specificity: AA 22-36, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-GPR120/FFAR4 (extracellular) Antibody (ABIN7043397, ABIN7044281 and ABIN7044282) is a highly specific antibody directed against an epitope of the mouse Free fatty acid receptor 4. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize FFAR4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7236163)

Blocking Peptide: (ABIN7236163)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for O3FAR1

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Alternative Name: FFAR4

Background:

Synonyms: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1

Description: Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis2-4.GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either Gαq or β-arrestin2,3.GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages1,2.

Gene ID: 107221

NCBI Accession: NM_181745

UniProt: Q7TMA4

Pathways: Hormone Transport