Histone 3 antibody (pSer28)

Catalog No. ABIN5706879

Supplier Product No.: 600-401-i83

The Rabbit Polyclonal anti-Histone 3 antibody (ABIN5706879) specifically detects Histone 3 in WB, IHC, IF, ChIP, FM and MA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Histone 3 antibody (pSer28) (ABIN5706879)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Product Details anti-Histone 3 Antibody

Binding Specificity: pSer28

Supplier Product No.: 600-401-i83

Supplier: Rockland

Purpose: Histone H3 phospho S28 Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H3.

Characteristics: Synonyms: rabbit anti-Histone H3 pS28 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87782, MGC87783, H3pS28

Purification: Anti-Histone H3 [p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H3 [p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 28 of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:150

Application Note: Anti-Histone H3 [p Ser28] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:150

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of serine 28 of H3 (H3 pS28) is a mitotic and immediate early gene marker. Although similar to phosphorylation on serine 10 of H3 in the role of chromatin condensation in mitosis and the ability to respond to external stimuli such as stress and mitogen activation, these two phosphorylation sites are independent and promote gene expression separately. Phosphorylation of S28 is modulated by MSK1, MSK2, and Aurora-B kinase. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones research.

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3