Histone 3 antibody (pThr11)

Catalog No. ABIN5706880

Supplier Product No.: 600-401-i76

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IHC, IF, ChIP, DB and FM. It exhibits reactivity toward Human.

Quick Overview for Histone 3 antibody (pThr11) (ABIN5706880)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-Histone 3 Antibody

Binding Specificity: pThr11

Supplier Product No.: 600-401-i76

Supplier: Rockland

Purpose: Histone H3 phospho T11 Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H3.

Characteristics: Synonyms: rabbit anti-Histone H3 pT11 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3pT11

Purification: Anti-Histone H3 [p Thr11] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H3 [p Thr11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Threonine 11 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H3 [p Thr11] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:50

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Background: Phosphorylation of H3 T11 is most abundant in the centromere, and initiates centromere assembly in mammalian cells. Dlk/ZIP kinase phosphorylates H3 T11, but may be affected by epigenetic modification itself during cellular interphase, which changes the rate of phosphorylation and de-phosphorylation of H3 T11. Other epigenetic modifiers also interact with H3 T11, including the histone acetyltransferase GCN5, which binds more readily to pT11 than unphosphorylated peptide. This binding affects downstream regulation of GCN5 genes and can contribute to transcriptional regulation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3