BAT3 antibody (Internal Region)

Catalog No. ABIN570700

This Goat Polyclonal antibody specifically detects BAT3 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for BAT3 antibody (Internal Region) (ABIN570700)

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAT3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-BAT3 Antibody

Binding Specificity: Internal Region

Purpose: BAT3 / BAG6

Sequence: PETNAPNHPS PAEY

Specificity: This antibody is expected to recognize both reported isoforms (NP_004630.3, NP_542433.1). Reported variants representing identical protein: NP_542434.1, NP_001092004.1, NP_542433.1 (isoform b).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PETNAPNHPSPAEY, from the internal region of the protein sequence according to NP_004630.3, NP_542433.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleoli staining in splenocytes. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments in Human Brain (Frontal Cortex) and Human Ovary lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any result

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Alternative Name: BAT3

Background: BAT3, HLA-B associated transcript 3, BAG-6, BAG6, D6S52E, G3, HLA-B associated transcript-3, large proline-rich protein BAT3, scythe

Gene ID: 7917, 224727, 94342

NCBI Accession: NP_004630, NP_542433