THBS3 antibody (C-Term)
Quick Overview for THBS3 antibody (C-Term) (ABIN570729)
Target
See all THBS3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- C-Term
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Purpose
- THBS3
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Sequence
- CNDTVPEDFE PFRRQ
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Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence CNDTVPEDFEPFRRQ, from the C Terminus of the protein sequence according to NP_009043.1.
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Isotype
- IgG
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Application Notes
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Immunohistochemistry: In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2 μg/mL.
Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Lung and in lysates of cell line A549 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the
Peptide ELISA: antibody detection limit dilution 1:32000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- THBS3 (Thrombospondin 3 (THBS3))
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Alternative Name
- THBS3
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Background
- THBS3, thrombospondin 3, MGC119564, MGC119565, TSP3, OTTHUMP00000033827
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Gene ID
- 7059, 21827
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NCBI Accession
- NP_009043
Target
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