Integrin beta 3 antibody

Catalog No. ABIN5707545

The Rabbit Monoclonal anti-Integrin beta 3 antibody has been validated for IHC (p). It is suitable to detect Integrin beta 3 in samples from Human.

Quick Overview for Integrin beta 3 antibody (ABIN5707545)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ITGB3-2166R

Product Details anti-Integrin beta 3 Antibody

Purification: Purified

Purity: Protein A affinity

Immunogen: Human recombinant protein was used as the immunogen for the recombinant Integrin beta 3 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titration of the recombinant Integrin beta 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Integrin beta 3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: Integrin beta 3 / ITGB3 / CD61

Background: Reacts with human Integrin beta 3, also called CD61. It associates with the α,V-chain (CD51) to form vitronectin receptor, or with the α,IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex