EpCAM antibody (Extracellular Domain)

Catalog No. ABIN5707556

This Rabbit Monoclonal antibody specifically detects EpCAM in IHC (p). It exhibits reactivity toward Human.

Quick Overview for EpCAM antibody (Extracellular Domain) (ABIN5707556)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-2041R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 100-224, Extracellular Domain

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas, in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]