CD99 antibody

Catalog No. ABIN5707579

This anti-CD99 antibody is a Rabbit Monoclonal antibody detecting CD99 in IHC (p). Suitable for Human.

Quick Overview for CD99 antibody (ABIN5707579)

Target: CD99

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDLA99-2R

Product Details anti-CD99 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Human protein was used as the immunogen for the recombinant CD99 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant CD99 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: CD99 is found on the cell surface of Ewing's sarcoma tumors and is positive in granulosa cell tumors. It is more expressed in malignant gliomas than in the brain, and such overexpression results in higher levels of invasiveness and lower rates of survival. Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma.[Wiki]