SMC5 antibody (Internal Region)

Catalog No. ABIN570758

The Goat Polyclonal anti-SMC5 antibody is suitable to detect SMC5 in samples from Human. It has been validated for ELISA, IF and FACS.

Quick Overview for SMC5 antibody (Internal Region) (ABIN570758)

Target: SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMC5 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SMC5 Antibody

Binding Specificity: Internal Region

Purpose: SMC5

Sequence: TSKHLEHKDN ELRQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TSKHLEHKDNELRQ, from the Internal region of the protein sequence according to NP_055925.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed bands at approx 100+150 kDa in HEK293 nuclear lysates after 1 μg/mL antibody staining (calculated MW of 129 kDa according to NP_055925.2). These bands were successfully blocked by incubation with the immunizing pept

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMC5

Target: SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))

Alternative Name: SMC5

Background: SMC5, structural maintenance of chromosomes 5, KIAA0594, SMC5L1, OTTHUMP00000063367, SMC5 protein, SMC5 structural maintenance of chromosomes 5-like 1

Gene ID: 23137, 226026, 293967

NCBI Accession: NP_055925