Nebulette antibody (Internal Region)

Catalog No. ABIN570759

This anti-Nebulette antibody is a Goat Polyclonal antibody detecting Nebulette in ELISA. Suitable for Human.

Quick Overview for Nebulette antibody (Internal Region) (ABIN570759)

Target: Nebulette (NEBL)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Nebulette antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-Nebulette Antibody

Binding Specificity: Internal Region

Purpose: NEBL / nebulette

Sequence: ELQRLKRTQE

Specificity: This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1, NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1, NP_001166955.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 30 kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/mL antibody staining. This protein has a calculated MW of 31.2 kDa according to NP_998734.1. T

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Nebulette

Target: Nebulette (NEBL)

Alternative Name: NEBL

Background: NEBL, nebulette, FLJ53769, LNEBL, MGC119746, MGC119747, bA56H7.1, LIM-nebulette, NEBL, actin-binding Z-disc protein, OTTHUMP00000019268, actin-binding Z-disk protein

Gene ID: 10529, 74103

NCBI Accession: NP_998734, NP_001166955