Involucrin antibody

Catalog No. ABIN5707639

This anti-Involucrin antibody is a Rabbit Monoclonal antibody detecting Involucrin in IHC (p). Suitable for Human.

Quick Overview for Involucrin antibody (ABIN5707639)

Target: Involucrin (IVL)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IVRN-2113R

Product Details anti-Involucrin Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Involucrin purified from human keratinocytes was used as the immunogen for the recombinant Involucrin antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant Involucrin antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background: Involucrin is a highly reactive, soluble, transglutaminase substrate protein present in keratinocytes of epidermis and other stratified squamous epithelia. It first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase thus helping in the formation of an insoluble envelope beneath the plasma membrane functioning as a glutamyl donor during assembly of the cornified envelope. [Wiki]