ICOS antibody (Internal Region)

Catalog No. ABIN570765

This anti-ICOS antibody is a Goat Polyclonal antibody detecting ICOS in IHC and ELISA. Suitable for Human.

Quick Overview for ICOS antibody (Internal Region) (ABIN570765)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ICOS antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ICOS Antibody

Binding Specificity: Internal Region

Purpose: ICOS

Sequence: KGGQILCDLT KTKGS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: KGGQILCDLTKTKGS

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Tonsil and Thymus. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 13 kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the c

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: ICOS, inducible T-cell co-stimulator, AILIM, CD278, MGC39850, activation-inducible lymphocyte immunomediatory molecule, inducible costimulator

Gene ID: 29851, 54167

NCBI Accession: NP_036224

Pathways: Cancer Immune Checkpoints