MUC16 antibody

Catalog No. ABIN5707666

This anti-MUC16 antibody is a Rabbit Monoclonal antibody detecting MUC16 in IHC (p). Suitable for Human.

Quick Overview for MUC16 antibody (ABIN5707666)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OCA125-2349R

Product Details anti-MUC16 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A recombinant partial protein was used as the immunogen for the recombinant MUC16 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant MUC16 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MUC16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16 / CA125

Background: The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs). Membrane-associated and secretory mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), 6-8, 11-13 and 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.