Desmoglein 1 antibody (Internal Region)

Catalog No. ABIN570770

The Goat Polyclonal anti-Desmoglein 1 antibody has been validated for IHC and ELISA. It is suitable to detect Desmoglein 1 in samples from Human.

Quick Overview for Desmoglein 1 antibody (Internal Region) (ABIN570770)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Desmoglein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Desmoglein 1 Antibody

Binding Specificity: Internal Region

Purpose: Desmoglein 1 / DSG1

Sequence: YTTSDTLKPS VHVHD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YTTSDTLKPSVHVHD, from the internal region of the protein sequence according to NP_001933.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skin showsstaining of the cellular membranes of keratinocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa, K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: DSG1

Background: DSG1, desmoglein 1, CDHF4, DG1, DSG, desmosomal glycoprotein 1, pemphigus foliaceus antigen

Gene ID: 1828

NCBI Accession: NP_001933

Pathways: Cell-Cell Junction Organization