Thyroglobulin antibody

Catalog No. ABIN5707705

This anti-Thyroglobulin antibody is a Rabbit Monoclonal antibody detecting Thyroglobulin in IHC (p). Suitable for Human and Rat.

Quick Overview for Thyroglobulin antibody (ABIN5707705)

Target: Thyroglobulin (TG)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TGB-1970R

Product Details anti-Thyroglobulin Antibody

Cross-Reactivity (Details): Not suitable for mouse

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Human thyroid follicular cells were used as the immunogen for the recombinant TG antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant TG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant TG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: TG / Thyroglobulin

Background: Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by the follicular cells of the thyroid and used entirely within the thyroid gland. Thyroglobulin protein accounts for approximately half of the protein content of the thyroid gland. Human TG (HTG) is a homodimer of subunits each containing 2768 amino acids as synthesized (a short signal peptide may be removed from the N-terminus in the mature protein).

The protein is a precursor of the thyroid hormones, these are produced when thyroglobulin's tyrosine residues are combined with iodine and the protein is subsequently cleaved. Each thyroglobulin molecule contains approximately 100-120 tyrosine residues, but only a small number (20) of these are subject to iodination by thyroperoxidase in the follicular colloid. Therefore, each Tg molecule forms only approximately 10 thyroid hormone molecules. [Wiki]

Pathways: Thyroid Hormone Synthesis