Filaggrin antibody (AA 198-288)

Catalog No. ABIN5707857

The Rabbit Monoclonal anti-Filaggrin antibody has been validated for IHC (p). It is suitable to detect Filaggrin in samples from Human.

Quick Overview for Filaggrin antibody (AA 198-288) (ABIN5707857)

Target: Filaggrin (FLG)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FLG-1957R

Product Details anti-Filaggrin Antibody

Binding Specificity: AA 198-288

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Amino acids 198-288 of human Filaggrin were used as the immunogen for this recombinant FLG antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant FLG antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant FLG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: Filaggrin

Background: Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Pathways: Sensory Perception of Sound, Stem Cell Maintenance