CD8 alpha antibody

Catalog No. ABIN5707907

This Rabbit Monoclonal antibody specifically detects CD8 alpha in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD8 alpha antibody (ABIN5707907)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDLA8a-2R

Product Details anti-CD8 alpha Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD8 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. [Wiki]

Pathways: TCR Signaling