UPF3A antibody (Internal Region)

Catalog No. ABIN570791

The Goat Polyclonal anti-UPF3A antibody has been validated for ELISA. It is suitable to detect UPF3A in samples from Human.

Quick Overview for UPF3A antibody (Internal Region) (ABIN570791)

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UPF3A antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-UPF3A Antibody

Binding Specificity: Internal Region

Purpose: UPF3A / RENT3A

Sequence: KETDKQKKIA EKE

Specificity: This antibody is expected to recognize both isoforms (NP_075387.1, NP_542418.1).

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KETDKQKKIAEKE, from the internal region of the protein sequence according to NP_075387.1, NP_542418.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 48 kDa band in lysates of cell lines HeLa and Kelly after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UPF3A

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Alternative Name: UPF3A

Background: UPF3A, UPF3 regulator of nonsense transcripts homolog A (yeast), HUPF3A, RENT3A, UPF3, OTTHUMP00000018789, UPF3 regulator of nonsense transcripts homolog A

Gene ID: 65110

NCBI Accession: NP_075387, NP_542418