Cadherin-16 antibody

Catalog No. ABIN5707925

This Rabbit Monoclonal antibody specifically detects Cadherin-16 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Cadherin-16 antibody (ABIN5707925)

Target: Cadherin-16 (CDH16)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KSCP2-2R

Product Details anti-Cadherin-16 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Cadherin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Cadherin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: Cadherin 16

Background: The CDH16 gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. Cadherin 16 consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. [Wiki]