GYPA
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant Glycophorin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Restrictions
For Research Use only
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative
Azide free
Storage
4 °C,-20 °C
Storage Comment
Store the recombinant Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Target
CD235a/GYPA (GYPA)
(Glycophorin A (GYPA))
Alternative Name
Glycophorin A
Background
Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also been designated CD235a. Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. [Wiki]