ISR-alpha antibody

Catalog No. ABIN5707973

Product Details anti-ISR-alpha Antibody

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ISR-alpha antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A recombinant partial protein from the extracellular region of the protein was used as the immunogen for the recombinant Insulin Receptor alpha antibody.

Clone: INSR-2277R

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Insulin Receptor alpha antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Insulin Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ISR-alpha

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Alternative Name: Insulin Receptor alpha (ISR-alpha Products)

Synonyms: CD220 antibody, HHF5 antibody, insulin receptor antibody, INSR antibody

Background: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular β, subunit and an extracellular α, subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.