CD27 antibody

Catalog No. ABIN5707991

This Rabbit Monoclonal antibody specifically detects CD27 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD27 antibody (ABIN5707991)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDLA27-1R

Product Details anti-CD27 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Human CD27 protein was used as the immunogen for the recombinant CD27 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant CD27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. CD27 transduces signals that lead to the activation of NF-kB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [Wiki]

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints