CD27 antibody (CD27 Molecule)

Details for Product anti-CD27 Antibody No. ABIN94051
Antigen
Reactivity
Human
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87
51
11
7
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1
1
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Host
Mouse
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37
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD27 antibody is un-conjugated
39
35
23
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6
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2
2
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2
2
2
2
2
2
2
2
2
2
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2
1
1
1
1
1
1
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1
Application
Flow Cytometry (FACS)
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75
75
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37
24
16
15
10
6
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Options
Immunogen Human peripheral blood lymphocytes
Clone LT27
Isotype IgG2a
Specificity The antibody LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
HLDA V, WS Code T T-CD27.01
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD27 (CD27 Antibody Abstract)
Background CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Pathways Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD27 Molecule (CD27) antibody (ABIN94051) Flow Cytometry analysis Surface staining of human peripheral blood cells with anti-h...