MYOD1 antibody

Catalog No. ABIN5708000

Product Details anti-MYOD1 Antibody

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this recombinant MyoD antibody.

Clone: MYOD1-2075R

Isotype: IgG kappa

Application Details

Application Notes: The optimal dilution of the recombinant MyoD antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MyoD antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD1 (MYOD1 Products)

Background: MyoD, or MYOD1, is one of the earliest markers of myogenic commitment. MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage. The effect of MyoD on satellite cells is dose-dependent, high MyoD expression represses cell renewal, promotes terminal differentiation and can induce apoptosis. Although MyoD marks myoblast commitment, muscle development is not dramatically ablated in mouse mutants lacking the MyoD gene. This is likely due to functional redundancy from Myf5 and/or Mrf4. Nevertheless, the combination of MyoD and Myf5 is vital to the success of myogenesis. [Wiki]

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development