NKX2-1 antibody

Catalog No. ABIN5708021

This Rabbit Monoclonal antibody specifically detects NKX2-1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for NKX2-1 antibody (ABIN5708021)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HBNK2-2R

Product Details anti-NKX2-1 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for the recombinant NKX2.1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant NKX2.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: NKX2.1 / TTF-1

Background: NKX2.1/TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1 reactivity is also seen in thyroid malignancies.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour