NKX2-1 antibody

Catalog No. ABIN5708025

The Rabbit Monoclonal anti-NKX2-1 antibody has been validated for IHC (p). It is suitable to detect NKX2-1 in samples from Human, Mouse, Rat.

Quick Overview for NKX2-1 antibody (ABIN5708025)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NX2-1-1855R

Product Details anti-NKX2-1 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for the recombinant NKX2.1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant NKX2.1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: NKX2.1 / TTF1

Background: NKX2.1/TTF1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. NKX2.1 reactivity is also seen in thyroid malignancies.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour