IKZF4 antibody (Internal Region)

Catalog No. ABIN570827

The Goat Polyclonal anti-IKZF4 antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect IKZF4 in samples from Human and Mouse.

Quick Overview for IKZF4 antibody (Internal Region) (ABIN570827)

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IKZF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IKZF4 Antibody

Binding Specificity: Internal Region

Purpose: IKZF4

Sequence: RPTFIDRLAN SLTKR

Specificity: This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IKZF4

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Alternative Name: IKZF4

Background: IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos

Gene ID: 64375, 22781

NCBI Accession: NP_071910