SPAG16 antibody (AA 139-152)

Catalog No. ABIN570832

This Goat Polyclonal antibody specifically detects SPAG16 in ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for SPAG16 antibody (AA 139-152) (ABIN570832)

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SPAG16 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SPAG16 Antibody

Binding Specificity: AA 139-152

Purpose: SPAG16 (aa139-152)

Sequence: KGVTELRTVG NVPD

Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_078808.3, NP_001020607.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KGVTELRTVGNVPD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173).

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPAG16

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Alternative Name: SPAG16

Background: SPAG16, sperm associated antigen 16, DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29, WD repeat domain 29, sperm-associated WD repeat protein

Gene ID: 79582

NCBI Accession: NP_078808, NP_001020607