FBXO4 antibody (Internal Region)

Catalog No. ABIN570843

This anti-FBXO4 antibody is a Goat Polyclonal antibody detecting FBXO4 in ELISA and IHC. Suitable for Human.

Quick Overview for FBXO4 antibody (Internal Region) (ABIN570843)

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FBXO4 Antibody

Binding Specificity: Internal Region

Purpose: FBXO4

Sequence: EHTSAVNKMF SRH

Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_036308.1, NP_277019.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EHTSAVNKMFSRH, from the internal region of the protein sequence according to NP_036308.1, NP_277019.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 48 kDa band in Human Heart ysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1 kDa

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FBXO4

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Alternative Name: FBXO4

Background: FBXO4, F-box protein 4, DKFZp547N213, FBX4, FLJ10141, F-box only protein 4, F-box protein Fbx4

Gene ID: 26272, 106052, 310363

NCBI Accession: NP_036308, NP_277019