Desmoplakin antibody (Internal Region)

Catalog No. ABIN570871

This Goat Polyclonal antibody specifically detects Desmoplakin in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Desmoplakin antibody (Internal Region) (ABIN570871)

Target: Desmoplakin (DSP)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Desmoplakin Antibody

Binding Specificity: Internal Region

Purpose: Desmoplakin (aa 1672 -1684)

Sequence: QESVKQAHLR NEH

Specificity: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QESVKQAHLRNEH

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: DSP

Background: DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I

Gene ID: 1832, 109620, 306871

NCBI Accession: NP_004406

Pathways: Cell-Cell Junction Organization